Applications of fluorophore-containing microbial growth media.

نویسندگان

  • W S Ramsey
  • E D Nowlan
  • L B Simpson
  • R A Messing
  • M M Takeguchi
چکیده

Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacteria. The emitted light from the gram-negative bacteria differed in wave-lengths from that of light emitted by colonies of gram-positive bacteria. The fluorescent intensity of colonies on complete 8-anilino-1-napthalene sulfonic acid agar supplemented with 1% of single substrates varied depending on the bacterial species, thus allowing the development of profiles used to identify 12 different species.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 39 2  شماره 

صفحات  -

تاریخ انتشار 1980